Players navigate through a three-dimensional space, interacting with objects and elements to progress through the game. The gameplay involves exploration and manipulation of various objects, allowing players to influence the environment and uncover hidden secrets.
The main objective of the game is to overcome obstacles and challenges that block progress. The player's actions determine the outcome, and the game presents a unique response to each decision. Super Mayo 64 can be played in a web browser without the need for a download or registration, allowing players to access the game directly online.